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Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 12, Issue 6 (2023)
Optimization of LD50, frequency and spectrum of chlorophyll mutation, efficiency and effectiveness of gamma rays in different cultivars of rice (Oryza sativa L.)
Author(s):
AH Jadhav, SS Desai, SG Bhave, AV Mane, SV Sawardekar, VV Dalvi and CP Bal
AH Jadhav, SS Desai, SG Bhave, AV Mane, SV Sawardekar, VV Dalvi and CP Bal
Abstract:
The present investigation entitled “Induction of genetic variability through gamma rays and assessment of variants by molecular markers in rice (Oryza sativa L.)” was carried with aim to create genetic variability by using different mutagenic treatments of gamma rays in rice cultivars Chakhao, Bangalya and Ghansal. The trials were carried out during kharif 2021 and Summer 2022 at Botany farm, Department of Agricultural Botany, College of Agriculture and molecular analysis was carried out in the laboratory of Plant Biotechnology Centre, Dr. B. S. Konkan Krishi Vidyapeeth, Dapoli.
The present investigation entitled “Induction of genetic variability through gamma rays and assessment of variants by molecular markers in rice (Oryza sativa L.)” was carried with aim to create genetic variability by using different mutagenic treatments of gamma rays in rice cultivars Chakhao, Bangalya and Ghansal. The trials were carried out during kharif 2021 and Summer 2022 at Botany farm, Department of Agricultural Botany, College of Agriculture and molecular analysis was carried out in the laboratory of Plant Biotechnology Centre, Dr. B. S. Konkan Krishi Vidyapeeth, Dapoli.
Decreasing trend was observed for percent germination in all mutagenic treatments with increased dose of mutagen under laboratory as well as under field condition. Similar trend was also recorded in germination percent, shoot length, pollen fertility and spikelet fertility. Considering laboratory and field observation on percent germination and other related parameters, LD50 dose was optimized. For chakhao and bangalya cultivars mutagenic treatment 300Gy, 0.20% and in case of ghansal cultivar 100 Gy doses were optimized as a LD50 dose.
During M1 generation, early flowering, late flowering, sterile spikelet mutants were recorded. Four types of chlorophyll mutations viz. Albina, Xantha, Chlorina and Striata, were noticed in all mutagenic treatments. Mutation frequency, mutagenic efficiency and mutagenic effectiveness were reduced as per the increased dose of mutagen.
Wide range of variation was observed in quantitative characters during M2 generation viz., days to fifty percent of flowering, maturity duration, plant height, number of tillers per plant, number of spikelets per panicle and grain yield per plant.
Pages: 4327-4333 | 168 Views 109 Downloads
How to cite this article:
AH Jadhav, SS Desai, SG Bhave, AV Mane, SV Sawardekar, VV Dalvi, CP Bal. Optimization of LD50, frequency and spectrum of chlorophyll mutation, efficiency and effectiveness of gamma rays in different cultivars of rice (Oryza sativa L.). Pharma Innovation 2023;12(6):4327-4333.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
