Vol. 12, Issue 6 (2023)

Author(s):
Anuj Rana, Kartikay Bisen, Ravi Kumar and Saurabh Kumar

Abstract:
Chickpea (Cicer arietinum L.), 2n=2x=14, which is a member of the Papilionaceae subfamily of the Leguminoceae family. The experiment was perform in Rabi season (2021-2023) at Rama University farm and Randomized Block Design (RBD) was followed. In present study treatment was taken as Trichoderma viride (T₁), Bacillus subtilis (T₂), Pseudomonas fluorescens (T₃), Garlic bulb extract (T₄) @ 5% and 10% concentration, Neem Leaf extract (T₅) @ 5% and 10% concentration, Onion bulb extract(T₆) @ 5% and 10% concentration, Mancozeb (T₇) @ 500 ppm and 1000 ppm, Carbendazim (T₈) @ 500 ppm and 1000 ppm, Untreated Control (T₉) and each treatment had replicated three times. In vitro analysis of Fusarium oxysporum f. sp. ciceris with different treatment after 7 days of inoculation the minimum radial growth was obtained at 1000 ppm concentration of Mancozeb (0.00 mm) and Carbendazim (0.00 mm) followed by Trichoderma viride (21.00 mm), Bacillus subtilis (24.90 mm), Pseudomonas fluorescens (26.60 mm), 10% concentration of Garlic bulb extract (29.70 mm), Neem leaf extract (37.60 mm) and Onion bulb extract (42.50 mm), while the maximum radial growth was seen in the control (83.50 mm). Mancozeb and carbendazim (1000 ppm concentration) showed the highest growth inhibition (100%) followed by Trichoderma viride (72.83%), Bacillus subtilis (70.12%), Pseudomonas fluorescens (67.16%), garlic bulb extract (64.43%), Neem leaf extract (54.97%) and Onion bulb extrac (49.00%). In field the minimum disease incidence was recorded on 60 days after sowing in T₇ (Mancozeb 0.1% concentration) was recorded 86% disease control followed by T₈ (Carbendazim 0.1% concentration) 84.5%, T₁ (Trichoderma viride 4g/kg) 80.5%, T₃ Pseudomonas fluorescens 4g/kg (79.5%), T₂ (Bacillus subtilis 4g/kg) 76.8%, T₄ (Garlic bulb extract 10% concentration) 72.0%, T₅ (Neem leaf extract 10%) 58.0% and T₆ (Onion bulb extract 10% concentration) 49.5%. Similarly the disease incidence was recorded lowest 0% in treatment T₇ (Mancozeb) and T₈ (Carbendazim), followed by T₁ Trichoderma viride (6%), T₂ Bacillus subtilis (8%), T₃ Pseudomonas fluorescens (9%), T₄ Garlic bulb extract (16%), T₅ Neem leaf extract (24%) and T₆ Onion bulb extrac (26%) while highest disease severity recorded in control showing 42% recorded 60 days after sowing.