Vol. 12, Issue 6 (2023)

Author(s):
RK Ranjan, Yamleshwar, Dinesh Rai and Subhashi Kumar

Abstract:
A total of 70 seed samples collected from 25 locations were tested for associated seed borne mycoflora by using standard blotter method, standard agar plate method and standard paper towel method. In standard blotter paper method, standard agar plate method and standard paper towel method, five mycoflora were identified based on spore morphology namely, Aspergillus sp., Alternaria triticina, Bipolaris sorokiniana, Fusarium moniliforme and Penicillium sp. In standard blotter paper method, average mycoflora recorded in Samastipur and Muzaffarpur districts seed samples were Aspergillus sp. (28.34%), Alternaria triticina (15.63%), B. sorokiniana (9.29%), Penicillium sp. (8.90%) and Fusarium moniliformae (6.54%), standard agar plate method, average mycoflora recorded in Samastipur and Muzaffarpur district seed samples were Aspergillus sp. (28.46%), Alternaria triticina (16.09%), B. sorokiniana (9.49%), Penicillium sp. (8.63%) and Fusarium moniliformae (6.19%) and in standard paper towel method the average mycoflora recorded in Samastipur and Muzaffarpur district seed samples were Aspergillus sp. (10.76%), Alternaria triticina (4.98%), B. sorokiniana (5.99%), Penicillium sp. (1.86%) and Fusarium moniliformae (2.30%). Fungi Aspergillus sp and Alternaria triticina was found predominant in standard blotter paper, standard agar plate and standard paper towel methods followed by Bipolaris sorokiniana, Penicillium sp and Fusarium moniliforme.