Vol. 12, Issue 6 (2023)

Author(s):
Asha Yadav, MS Dalal, Manuj Saini, Prexha Kapoor, Dev Vart, Sonu Langaya, Amit, Harsh Deep and Kiran

Abstract:
The extent of genetic variability for grain yield and its attributing traits in forty inbred lines were evaluated at Bajra Section (G&PB), CCSHAU, Hisar. Significant differences were revealed by the ANOVA among the evaluated inbreds for every parameter studied. DNA was extracted for molecular marker analysis. Sixteen polymorphic markers were identified which were dispersed throughout the pearl millet genome. A total of 88 alleles were detected, collectively yielding SSR profiles for forty inbred lines. The average number of SSR alleles per locus was 5.5 with a range of 2 (ICMP 2084 and XCVMP 0018) to 16 (PSMP 2233). PIC values of various SSR loci across the 40 inbred lines ranged from 0.16 (ICMP 3088) to 0.94 (PSM 2233) with an average of 0.59 locus. It is significant to note that 6 out of 16 SSR loci namely PSMP 2233, PSMP 2008, PSMP 2263, ICMP 3050, PSMP 2274 and ICMP 3017 revealed PIC values above 0.70, can be considered highly useful for differentiation of Pearl millet inbred lines. The UPGMA cluster tree analysis led to the grouping of 40 inbred lines in eight major clusters. Clustering pattern revealed that cluster 3 and cluster 7 were the largest consisting of 10 inbred lines each. The inbred line H78/711 and TCP-10-110 grouped in cluster I and PT-1-10-1002 grouped in cluster II were similar on morphological characterization and molecular characterization leading to confirmation of these genotypes in same group.