Vol. 12, Issue 7 (2023)

Author(s):
Biswajit Sahoo, Sandeep Bhandarkar and Ramlakhan Verma

Abstract:
Rice (Oryza sativa L.) is one of the major food crops, feeding more than half of the world's population. Major aim of rice breeding program is to enhance the yield potential by utilizing genetically diverse parent. The study was conducted in Kharif 2016 and Rabi 2016-17 at the Research and Instructional Farm, Indira Gandhi Krishi Vishwavidyalaya (IGKV), Raipur, Chhattisgarh and Kharif 2017 at Research Farm of National Rice Research Institute (NRRI), Cuttack, Odisha (India). 10 rice parental genotypes, Chandrahasini, Samleshwari, Durgeshwari, IC-134022, IC-388728, IC-389860, IC-390376, IC-548384, Indira Barani Dhan1, IRHTN-105 and seven F3-F4 populations were taken for the study. Two hundred eighty-eight microsatellite primers (288 SSR and 38 gene based markers) were individually assayed on DNA of the parental genotypes (IC-548384, Chandrahasini, IC-390376, Samleshwari, IC-134022, Durgeshwari, and IC-388728, IC-389860) to reveal polymorphisms. Of these primers, 32 markers showed polymorphisms were utilized for trace the allelic contribution of the parental lines to their progeny; and drought and yield related specific genes. Total 32 segregating loci (95.65%) showed Mendelian pattern in segregation at p = 0.05 and p = 0.01, all are fit to a 1: 2: 1 ratio (Table 1). Distorted segregation ratios were found for markers RM-11846 ((IC-548384 X Chandrahasini) with χ2 = 6.456, (IC-390376 X Samleshwari) with χ2 = 10.75 & (IC-134022 × Durgeshwari) with χ2 = 6.78), RM-20773 ((IC-389860 X Samleshwari) with χ2 =6.087) and qDT-3850 (IC-548384 X Chandrahasini) with χ2 =12.432) at p<0.05 and p.01 probability level in the F3 population.