Vol. 12, Issue 7 (2023)

Author(s):
Pravas Ranjan Sahoo, Pashupathi M, Sreenila VV, Jyochhna Rani Sahoo, Meeta Saxena and Ajay Kumar

Abstract:
Salmonella typhimurium is one important intracellular, gram negative, non-spore forming pathogen causing food poisoning in human worldwide. During its pathogenesis, it encounters various anaerobic conditions, which is mediated by different regulators. NarL, narG, ssrB and FNR are important anaerobic regulators; affect the pathogenesis in the host cell. So there is a need of physiochemical and structural characterization of these proteins. So in this study, these anaerobic regulators proteins were characterized physiochemically by EXPASY Protoparam bioinformatics tool. Further the secondary and three dimensional structures were predicted through GORIV and SWISS Homology Modeling software tool respectively. All these proteins were found as stable, soluble, hydrophilic in nature. Further, NarL, ssrB and FNR showed a greater number of alpha helixes followed by random coils where as NarG contains large number of random coils. The three-dimensional structure of all NarL, NarG, ssrB and FNR showed that all proteins exist in monomer with RMSD value in least difference. So this study will provide a better platform for researcher to develop suitable therapeutics against these anaerobic regulators in Salmonella typhimurium infection.