Vol. 12, Issue 7 (2023)

Author(s):
Nazia Anjum, Sheetal Dogra, RK Pandey, Pankaj Pandotra, Nomita Laishram, Arvinder Singh, Subash Kashyap and Ali Haider Shah

Abstract:
The present investigation utilized different explants namely shoot tips, nodal segments and internodal segments of chrysanthemum cv. “Maghi White” for sterilization with different concentrations of mercuric chloride with respect to variations in time durations. It revealed that treatment with 0.1% mercuric chloride for 1 minute duration for shoot tip explants and that for 3 minutes duration for nodal and internodal segments was found best for maximum survival of the explants. For rooting, MS medium supplemented with IBA (1.00 mg/l) exhibited best results. And earliest root emergence (11.28 days), root length (5.53 cm) and percent rooting (85.18) were obtained on this medium. Potting mixture containing 75% Cocopeat + 25% Perlite was found most suitable for hardening of in vitro raised plantlets.