Vol. 12, Issue 7 (2023)

Author(s):
Shinde Umesh Dnyaneshwar, Arpita Mahobia, Navnath Gaikwad, Prafull Jalamkar, Amit Thakare, Toshy Agrawal and AS Kotasthane

Abstract:
Microorganisms play an essential role for nutrition management, disease prevention, and to promote plant growth. By eradicating plant diseases, the mycorrhizal fungi, some plant-growth-promoting rhizobacteria (PGPR), and fungi like Trichoderma spp. and Piriformospora indica can encourage plant growth. Some strains of Trichoderma that are rhizosphere-competent directly influence plants by increasing their capacity for growth, nitrogen uptake, and fertiliser utilisation. The focus of the current study is on the effects of ten different Trichoderma spp. (IRRI-1, IRRI-2, IRRI-3, IRRI-4, IRRI-5, IRRI-6, TH3 TV12 T14 94a) on the root system morphology of four rice varieties (IR-64, Sahbhagi dhan, RRF-75, and Indira barani dhan-1). The rice genotypes IR64 and RRF75 with isolate T14, Sahbhagi dhan with IRRI5, and Indira barani dhan with TV12 all showed the maximum root length. Following treatment with isolates IRRI-6, IRRI-4, IRRI-6, and IRRI-5, respectively, maximum shoot lengths were seen in IR-64, Sahbhagi dhan, RRF-75, and Indira Barani dhan. Spectrophotometric analysis of these isolates revealed a range of responses for the ACC deaminase activity test; isolate TV12 displayed the greatest activity (10.46 g/ml). Additionally, distinct gene-specific primers were used for the PCR-based amplification of several Trichoderma isolates. This research investigation will encourage breeders to select the desired genotypes on the basis of root architecture for drought tolerance.