Vol. 12, Issue 7 (2023)

Author(s):
Vaishanavi Zade, Kisan B, Ayyangouda Patil, Pampanna Y, Nagesha N Sreedhara JN and Sharanbasappa Yeri

Abstract:
The leafy vegetables requires biofertilizers for cultivation, faster and better growth. The utilization of beneficial microbes as a biofertilizer has become major source of nutrient supply for the sustainable crop production. Chlorella variabilis, a unicellular photosynthetic green algae, is an intracellular photobiont isolated from UAS, Raichur in BG II media by providing enriched 10% of carbon dioxide and grown under 12h light and dark period under room temperature. On spray to Sabaski as soon as three leaf stage of germination at different dosage (control, 10 ml/l,15 ml/l, 20 ml/l, 25 ml/l, 30 ml/l, 35 ml/l, 40 ml/l and Nyntinol 2 g/L) to know the growth and other biochemical parameters were recorded at harvest. The results showed highest plant height (25.6 to 30.2), chlorophyll content (28.6 to 53.5%), number of leaves (25 to 32) and weight of the plant (6.9 to 8.0 g), and the highest moisture content was 85.06 and 87.46, ash content 2.72 and 3.05, crude fat content 0.85 and 0.96, crude fiber 2.05 and 2.43, crude protein 2.86 and 3.04 and carbohydrates content 7.83 and 8.42 for the treatments T₁ (control), T₆ (30 ml/L) and T₈ (40 ml/L) respectively was recorded. The study showed that fast growing culture (ACR 5) with high contents of Lysine (2.49%), Tryptophan (0.98%), Iron (66.73 mg/kg), Zinc (60.22 mg/kg) on sabaski at T₆ (30ml/L), T₈ (40 ml/L) show increase in production by 20% physiological growth at harvest. Hence the treatment of 30 ml/L can be effectively utilized for the field spray of microalagal culture (SCP) to obtain higher vegetative growth.