Vol. 7, Issue 6 (2018)

Author(s):
Sadaf Sakeena, Rafiqul Islam, Qumaila Sakeena, MD Moin Ansari, Arjuma Khatum, Asloob Malik, FA Lone and BA Moulvi

Abstract:
This study was undertaken to study the alterations in morphological and progesterone in the blood serum, follicular fluid and corpus luteum of ewes with or without uterine infection. The uterine infection was diagnosed on the basis of white side test, pH of uterine washings and endometrial cytology. The reproductive stage was determined on the basis of the functional structures present on the ovaries. Both infectious and normal ewes were further sub divided into Follicular infectious (FI), Luteal infectious (LI), Follicular normal (FN), Luteal normal (LN) and Acyclic normal (AC). Serum was collected from the clotted whole blood and preserved at -20ºC till further processing. The mean pH of the uterine secretions obtained from ewes suffering from uterine infection was significantly higher (P<0.001) than normal ewes. The ovarian weight in the Follicular and Luteal group was higher than the Acyclic ewes. The total number of follicles and number of large follicles was significantly higher in follicular group than the other groups. The serum progesterone concentration (ng/ml) was significantly higher (P<0.05) in Luteal group of ewes (LN and LI) as compared to Follicular and Acyclic group of ewes. The mean follicular progesterone concentration was significantly higher (P<0.001) in luteal group of ewes (LI and LN) and Follicular infectious groups as compared to Follicular normal and Acyclic group of ewes.