Vol. 12, Issue 7 (2023)

Author(s):
Shivanshu Garg, Vipin Hallan, Pooja Nain and Nageswer Singh

Abstract:
The construct has been prepared for generating the silent lines of the model plant Nicotiana benthamiana. F-box Kelch is an important protein that associates with the viral proteins and is an important factor that helps in the establishment of viral infection. A conserved portion of the gene was amplified in sense and antisense orientation and in a sequential manner it was cloned into the silencing vector pSAT4. The construct development was ascertained with the help of restriction digestion and sequence determination. The construct is ready to be transformed into plants. For the biochemical analysis, purified Cucumber mosaic virus (CMV) was inoculated at their 3-4 leaf stage and infection was confined by symptom development. Both infected and healthy plants were subjected to biochemical analysis of the phenolic compounds (total phenols, simple phenols, Ortho-dihydric phenols and bound phenols), alkaloids and total chlorophyll and carotenoids. It was observed that the total phenol content was increased in the infected plant in comparison to the healthy leaves. However, total chlorophyll and carotenoids were reduced in infected plants as compared to healthy leaves. This is expected as virus infection leads to loss of chlorophyll as reflected by mosaic symptoms that the virus produces.