Vol. 12, Issue 7 (2023)

A quick, effective analytical technique was developed and validated to determine thiamethoxam residues in fresh and processed (mushroom in brine and dry mushroom powder) button mushroom by adopting modified QuEChERS method in LC-MS/MS. Various parameters such as linearity, limit of detection, limit of quantification, precision, and accuracy were considered to validate the modified method. The linearity of the calibration curve was established and shown good relationship with the R2 value 0.998 whereas LOD and LOQ was 0.003 and 0.01 µg/g respectively. Mean recoveries of thiamethoxam were ranged 89.30 to 108.3, 95.96 to 116.37 and 97.17 to 114.26% at spiking level from 0.01 to 0.1 µg/mL whereas RSD values ranged from 4.04 to 8.31, 5.67 to 8.70 and 3.38 to 9.97% for fresh button mushroom, button mushroom in brine and dry button mushroom powder, respectively. The validated method was used to monitor the mushroom samples. The results revealed that, there were no residues of thiamethoxam insecticide in any samples.