Vol. 12, Issue 7 (2023)

Author(s):
Nisha Thakur, Shamsher Alam, Aradhana Singh Paikra and Gopal Krishna Awadhiya

Abstract:
Alternaria lini caused linseed blight is a highly destructive pathogen. The linseed plant's aerial parts, including its leaves, stem, and pods, are all susceptible to the disease that afflicts it. The purpose of this investigation is to determine the impact that solid and liquid media have on the rate of growth, the number of spores produced, and the amount of mycelial dry weight. Result depicted that maximum mycelial growth observed in PDA (89.33 mm) followed by Potato+ CaCO₃ (86.67 mm), Czapek dox agar (78.00 mm). Minimum mycelial growth observed in Rose Bengal agar (48.00 mm). Colony color varied from dark gray, gray and light gray. Colony characters fluffy, cottony, smooth texture with circular or irregular growth pattern and thickness of mycelium thick, thin and very thin. Sporulation was found excellent in PDA medium. In case of broth media maximum mycelium dry weight was obtained in Potato dextrose broth (236.67 mg) followed by Potato + CaCO₃ broth (133.33 mg), Asthana and Hawker’s broth (123.33 mg), Czapek dox broth (120.00) and Ashby broth (106.67). Minimum mycelial dry weight was obtained in Rose Bengal broth (4.33 mg) followed by Richard’s broth (8.67 mg). According to the findings, the best medium for the growth and sporulation of Alternaria lini was Potato dextrose Agar, followed by Potato +CaCO₃ and Czapek dox agar. Furthermore, the best broth medium for the production of biomass by Alternaria lini was Potato dextrose broth, followed by Asthana and Hawker's broth.