Vol. 12, Issue 7 (2023)

Author(s):
Suman Verma, Sanjay Kumawat, Surendra Kumar Badasara, Mayank Rawat and Salauddin Qureshi

Abstract:
Brucella abortus is globally distributed facultative intracellular pathogen which cause significant economic losses in livestock and more often it is a neglected zoonotic threat for humans. Opsonophagocytosis mediated through host macrophages plays a pivotal role in destruction of threats inside host body, but its importance in recovery from infection with intracellular pathogens is still unclear. The main aim of this study was to access the role of B. abortus specific immune serum and interferon-gamma in post phagocytic clearance B. abortus through the flow cytometric measurement of reactive oxygen species produced by mice peritoneal macrophages. The study was conducted using mice peritoneal macrophages in vitro. Cultured macrophages were infected with opsonized and unopsonized B. abortus S19 (Vaccine strain) and S544 (virulent strain). Post-phagocytic clearance was measured in terms of extant of oxidative burst. Macrophages has undergone robust oxidative burst regardless of opsonized or unopsonized Brucella, measured by dihydrorhodamine 123. However, high rate of oxidative burst was observed in IFN-γ primed macrophages infected with opsonised strain.