Vol. 12, Issue 8 (2023)

Author(s):
Pema Wangchu, Tapor Pakpu and Ige Kamduk

Abstract:
sssssssssAgaricus bisporus germplasm consisting of popularly grown strains in India and some other collections was evaluated for identification of parental lines for single spore selection. In the current experiments, seven test strains of Agaricus bisporus were used, and they were cultivated on five different solid media. After 21 days of inoculation, radial growth of various test strains was recorded in four replications for each treatment. Strain A-15 developed a colony with a maximum diameter of (83.45 mm) on malt extract agar medium. Belgium-2 (check) strain measured a minimum (26.55 mm) colony diameter on oat meal agar medium. Agaricus bisporus test strains were cultured for 21 days at a variety of temperatures between 10 and 35 °C. Maximum growth of all the test strains including check (Belgium-2) was recorded at 25 °C (69.56 mm). Minimum growth of all the test strain including check (Belgium- 2) was recorded at 35 °C (11.79 mm). Temperature of 25 °C was found the best for the growth of all the test strains. Maximum length of cap was observed in strain A-15 (5.05 cm) followed by strain U-3 (4.78 cm), S-11 (4.28 cm) and Delta-N (4.05 cm) and minimum was taken in strain Belgium- 2 (check) (3.15 cm). Among the various test strains U-3 yielded maximum 15.08 kg/100 kg of compost. Strain A-15 having best desirable morphological characteristics with highest length of stalk 3.45 cm, width of stalk 3.03cm width of mushroom cap 4.73cm and length of mushroom cap 5.03 cm.