Vol. 12, Issue 8 (2023)

In tropical nations, Pleurotus ostreatus is regarded as a suitable alternative source of protein-rich diet. The ability of Pleurotus to concurrently produce fruiting bodies and reduce or degrade harmful chemicals found in the substrate is equally impressive. This research's goal was to examine how cultivating Pleurotus ostreatus was affected by various caffeine and yeast concentrations. P. ostreatus strain AVT-PP-22-102, also known as P. ostreatus, was created on a PDA using various concentrations of caffeine and yeast. Based on optimal mycelial growth, caffeine and yeast extract play a vital role in the development of Pleurotus ostreatus. The maximum radial mycelium growth (mm) was recorded maximum 8.15 mm, 13.15 mm, 19.25mm and 25.42 mm) in T₈ at 24, 48, 72 and 96 hours followed by T₇ (7.72 mm, 12.47 mm, 18.42 mm and 22.5 mm), T₁ (6.45 mm, 9.7 mm, 15.7 mm and 18.5 mm), T₂ (5.45 mm, 8.45 mm, 14.35 mm and 18.15 mm), T6 (4.55 mm, 9.55 mm and 14.85 mm and 17.12 mm), T₅ (3.47 mm, 8.47 mm, 10.47 mm and 12.35 mm) and T³ (2.25 mm, 5.25 mm, 10.25 mm and 10.75 mm). T₄ have the lowest radial growth recorded among all the treatment which gave 0.95 mm, 3.7 mm, 5.5 mm and 6.5 mm at 24, 48, 72 and 96 hours respectively.