Vol. 12, Issue 8 (2023)

Author(s):
Ganesh, Ramachandra B, Phaniraja KL, Venkatesh M and Prabha R

Abstract:
Bovine mastitis isolates of 45 numbers from positive milk samples of California Mastitis Test (CMT) in and around Bengaluru area were obtained from the Department of Veterinary Microbiology, Veterinary College, Bengaluru in nutrient broth. Isolates were purified using streaking technique among them 42 number isolates showed the uniform remaining 3 isolates revealed with circular colony morphology on to the streaked plates. Cell morphology of the 45 isolates upon subjecting to the gram staining showed 40 number with purple color with cell arrangement cocci in chains (22), cocci in bunches (18) isolates assigned with Gram positive streptococci, staphylococci respectively. while the remaining 5 isolates showed pink color and short rods under the oil immersion microscope. Thus, in the present study, Gram positive bacterial pathogen were predominant fallowed by the Gram-negative mastitis pathogens.