Vol. 12, Issue 8 (2023)

Author(s):
PN Madavi, NG Dhurve and SB Tambe

Abstract:
The present investigation was carried for molecular variability of Xanthomonas axonopodispv. citricausing citrus canker. Eight isolates of Xanthomonas were obtained on NA medium from the canker infected leaves collected from different places.The variability was studied among the eight isolates of Xanthomonas axonopodis pv. citri by using 7 ISSRprimers i.e.ISSR8, ISSR12, ISSR817, ISSR820, ISSR 827, ISSR841, ISSR57101. 7 primers produced 22 scorable bands with an average of 3 bands per primer. Out of 22 bands, 19 bands were polymorphic and level of polymorphism was 68%.The UPGMA analysis showed that Isolate Xac6 (Pune) had higher value of similarity coefficient (0.91) with Xac7(Rahuri), whereas Xac1(Akola),had lower value of similarity coefficient (0.50) with Xac8(Dapoli).