Vol. 12, Issue 8 (2023)

Author(s):
Hemangini Chaudhari and YM Shukla

Abstract:
WRKY is a member of an important plant specific major transcription factor family showing expression in majority of plant species. Little is known about expression of WRKY transcription factors in pearl millet. So the objective of present study is to analyse expression level of WRKY transcription factors during different developmental stages. Leaves and roots sample of two genotypes were studied. Out of four reference genes namely Actin, PP2A, TIP-41 and UBQ 5, Tip-41 was found most stable reference gene. Relative quantification of WRKY transcription factors was carried out for four WRKY transcription factor genes which revealed an alteration in expression level at different developmental stages and tissue. Present study is helpful to understand the importance of WRKY transcription factors during developmental stages. Detailed expression study of WRKY genes specific for particular gene or specific conditions may provide an important insight to unravel unknown mechanisms of plant growth and development.