Vol. 12, Issue 8 (2023)

Author(s):
Manisha Solanki, Dr. Pramod Borkar, JJ Kadam and PP Salvi

Abstract:
The Alternaria blight is an important disease among the different cultivated and wild hosts. The diseased leaves of different plants species showing typical symptoms of Alternaria leaf blight were collected from different cultivated and wild hosts from different locations. Total 12 cultures of Alternaria were isolated and proved their pathogenicity. Pure culture of the fungus so obtained was sub cultured on PDA slants and incubated at 27 ± 2 °C. The growth of 12 isolates of A. alternata were studied on 6 different media (three Synthetic & three non-synthetic) namely Potato dextrose agar medium, Oat meal agar medium, Richard’s medium, Asthana & Howker’s medium, Sabourauds agar medium and V8 juice agar medium. Each treatment was replicated thrice. On the basis of linear growth, the best medium for the growth of the fungus was identified. The cultural characteristics, viz., topography, colour of colony, growth pattern, type of margin were recorded periodically. The variation was observed in cultural characteristics such as colony colour, growth pattern and zonation.