Vol. 12, Issue 9 (2023)

Author(s):
Kusum Thakur, RS Netam, Anurag Kerketta, AK Thakur, DP Singh, Aayush Singh, Dainish Pujari, Shruti Yadu, Shubham Kale and Kanhaiya Sai

Abstract:
Brown leaf spot of rice is found to be one of the most destructive diseases of rice. The study was carried out to determine the effect of different temperatures, pH and media on growth and sporulation of Helminthosporium oryzae under in vitro condition. Among different temperature range studied, maximum mycelium growth with 28.66 mm, 54.62 mm and 83.60 mm was observed at 30 °C on 48, 96 and 144 hours after inoculation with sporulation (6.1×10⁴ cfu/ml) followed by 25 °C, 35 °C and 20 °C. Maximum mycelium growth 21.80 mm, 43.46 mm and 86.52 mm was recorded at pH7.0 after 48, 96 and 144 hours of incubation with recorded sporulation (6.7×10⁴ cfu/ml) followed by pH 6.0. Among nine different cultures media, maximum mycelium growth recorded at Paddy leaf extract medium with 27.22 mm, 64.60 mm, 90.00 mm after 48, 96 and 144 hours with recorded sporulation of 7.2×10⁴ cfu/ml followed by Oat meal medium and Corn meal agar medium.