Vol. 12, Issue 8 (2023)

Author(s):
KL Nikhila, C Aswath, PT Srinivas and P Nandeesha

Abstract:
In the present paper, a procedure for rooting of in vitro plantlets raised from capitulum culture of gerbera is described. Subsequent to in vitro regeneration and multiplication, the plantlets are transferred to a rooting medium, initiating an examination of their rooting behaviour and pertinent parameters. Full strength MS media supplemented with 3% sucrose, 0.4% gellan gum and 1000 ppm NAA has resulted in 99.6% of rooting. Earliest root initiation (9.20 days) and maximum root length (4.71 cm) were also recorded in treatment T₅ i.e., full strength MS media supplemented with 3% sucrose, 0.4% gellan gum and 1000 ppm NAA. After 30 days the rooted plantlets are transferred to jiffy plugs for primary hardening and acclimatisation.