Vol. 12, Issue 9 (2023)

Author(s):
Vikas Verma and Rama Shankar Shukla

Abstract:
This study aims to compare the protein profiles and quantities of different wheat genotypes of Triticum aestivum and Triticum durum using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The researchers hypothesize that the protein profiles and quantities would differ among different genotypes and the differences may be associated with the end-use quality of the wheat. The study used seven genotypes of T. aestivum and eighteen genotypes of T. durum grown under normal conditions. The albumin-globulin extraction, gliadin extraction, and extraction of soluble glutenin were done to sequentially extract defatted wheat flour to study the protein profiles. The results of this study could provide valuable information for wheat breeders and researchers to develop new wheat cultivars with improved end-use quality.