Vol. 12, Issue 9 (2023)

The current study was carried out in a completely randomized design at the Department Plant Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist. Akola (M.S.) during the academic year 2015–2017 with the aims of producing disease and virus free plantlets, standardizing explant type and media combinations influenceable for tissue culture, and in vitro micro propagation of Santalum album.

The highest survival percentages were 70.00% and 68.33% for nodal explants after two and three weeks of therapy with MS media supplemented with BAP 1 mg/l + kinetin 1 mg/l, respectively. After two weeks, the nodal explants were inoculated with MS + BAP + Kin @ 1 mg/l for the start of the shoot bud. The highest survival rate, 63.33 percent, was noted. The maximum percent of shoot bud initiation, or 61.66 percent, was achieved after three weeks of inoculation on MS + BAP + Kin @ 1 mg/l each. The highest multiple shot survival rates were seen for multiple shoot induction after three and six weeks of therapy with MS media incorporated with BAP 3 mg/l + Kin 1 mg/l + ADS 2 mg/l, after three weeks (5.06) and six weeks (2.81).