Vol. 12, Issue 9 (2023)

Author(s):
Jnapika KH, Dr. Sarvamangala Cholin S, Dr. Kulapati H, Dr. SG Gollagi, Dr. Sanjeevaraddi GR and Basavaraj Padashetti

Abstract:
The current study was conducted at the College of Horticulture in Bagalkot, Karnataka, between 2017 and 2019. A Randomized Complete Block Design was used to set up the experiment, and twelve cultivars were duplicated three times. Horticulturists have traditionally used morphological and physiological descriptors to identify different varieties of fruit crops. Using the allele sizes of nine SSR markers acquired from previously described Annona species, the genetic diversity of the 12 Annona cultivars was evaluated (Larranaga and Hormaza, 2015). Nine microsatellite markers were used to identify a total of 21 alleles from the nine SSR manufacturers that were evaluated across 12 Annona cultivars in the current investigation.In the current study, a total of 11 RAPD markers that were evaluated across 12 Annona cultivars amplified a total of 28 loci. Additionally, to determine how closely related these 12 cultivars were genetically, they were subjected to phylogenetic analysis using the pair-wise genetic matrix generated by RAPD markers. The results were presented in Figure 2 using an unrooted neighbour joining tree and the Mega7.0 software. The findings of the molecular analysis had shown that the genetic diversity of cultivars varied. Twelve Annona cultivars under investigation have been successfully characterized using the SSR and RAPD markers. The findings showed that SSR markers are co-dominant and highly reproducible. The significance of SSR markers in the molecular characterisation of Annona cultivars is indicated by this.