Vol. 12, Issue 9 (2023)

Present investigation aimed, to produce disease and virus free plantlets, by using sandalwood seeds on different media combinations amenable for tissue culture, to in vitro micro propagation of Santalum album was conducted in Completely Randomized Design at Centre of excellence in Plant Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist. Akola (M.S.) during the academic year 2015-2017.

Seed germination when MS incorporated with sucrose 3%without GA₃ and MS 3%, 4%, and 6% of sucrose with GA₃ 1mg/l was tried the highest seed germination was observed (86.66) when MS 3%+ GA₃ 1 mg/l was used. The explants derived from in vitro grown seedling explants were (shoot, epicotyl, hypocotyl) inoculated on 8 different media combination. The highest survival percentage was recorded in treatment MS + BAP 2 mg/L + NAA 0.4 + IBA 0.4% in case of shoot (91.66%), epicotyls (50%), and hypocotyl (33.33%).