Vol. 12, Issue 9 (2023)

Author(s):
P Sowmya, A Sankari, A Thanga Hemavathy, H Usha Nandhini Devi, K Iyanar, L Pugalendhi and M Djanaguiraman

Abstract:
The experiment was carried out in the department of Vegetable Science, TNAU, Coimbatore with an objective to determine the LD₅₀ value and the effect of mutagen on germination percentage, seedling length and seedling vigour index with respect to varying doses of Gamma rays and Ethyl methane sulfonate. The seeds of HA-3 were treated with six doses of gamma rays viz., 100 Gy, 150 Gy, 200 Gy, 250 Gy, 300 Gy, 350 Gy and six concentrations of Ethyl Methane Sulfonate viz., 10 mM, 20 mM, 30 mM, 40 mM, 50 mM and 60 mM. The treated seeds were sown in protray and sand tray which was placed in the germination chamber to fix the lethal dose value (LD₅₀) by using probit analysis. Based on the values obtained, it is concluded that the treatment 277.678 Gy of gamma irradiation and 41.541 mM of EMS concentration was identified as LD₅₀ value.