Vol. 12, Issue 9 (2023)

Author(s):
K Anbu Kumar, TV Meenambigai and P Devendran

Abstract:
Complete inactivation of porcine circovirus 2 (PCV2) at 37 °C using binary ethylene imine (BEI) at a final concentration of 0.1, 0.25, 0.5, 1.0, 1.5 and 3.0 mM was observed at 14, 14, 12, 10, 10 and 8 hours respectively, with inactivation rate ranging from 0.53 to 1.05 log₁₀/hour. At 25 °C the complete PCV2 inactivation was observed at 28, 28, 24, 24, 20 and 12 hours using BEI at a final concentration 0.1, 0.25, 0.5, 1.0, 1.5 and 3.0 mM, respectively, with inactivation rate ranging between 0.2 - 0.79 log₁₀/hour. The “total inactivation time” for completely inactivating the infectivity of 100 mL of 10^6.3 TCID₅₀/mL PCV2 with 3 mM BEI at 25 °C was determined as 36 hours.