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Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 12, Issue 9 (2023)
Cloning of eye pigmentation gene white from the oriental fruit fly, Bactrocera dorsalis (Hendel) (Tephritidae: Diptera) and CRISPR/Cas 9 based in vitro restriction assay of different sgRNAs
Author(s):
Sangeeta Dash
Sangeeta Dash
Abstract:
Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is a destructive pest causing tremendous economic losses. The present study was, aimed at validation of genetic targets for genetic biological control of this notorious pest via CRISPR/Cas 9 mediated genome editing. Gene white (2040 bp) crucial for melanin pigmentation and cuticle sclerotization was cloned. Further, off-target minimised guide RNA for white (sg 360) was synthesised in vitro. The designed gRNAs were used for restriction assay to check the cleavage efficiency of sgRNAs.
Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is a destructive pest causing tremendous economic losses. The present study was, aimed at validation of genetic targets for genetic biological control of this notorious pest via CRISPR/Cas 9 mediated genome editing. Gene white (2040 bp) crucial for melanin pigmentation and cuticle sclerotization was cloned. Further, off-target minimised guide RNA for white (sg 360) was synthesised in vitro. The designed gRNAs were used for restriction assay to check the cleavage efficiency of sgRNAs.
Pages: 2541-2545 | 122 Views 67 Downloads
How to cite this article:
Sangeeta Dash. Cloning of eye pigmentation gene white from the oriental fruit fly, Bactrocera dorsalis (Hendel) (Tephritidae: Diptera) and CRISPR/Cas 9 based in vitro restriction assay of different sgRNAs. Pharma Innovation 2023;12(9):2541-2545.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
