Vol. 7, Issue 7 (2018)

Author(s):
Affy Mataphouet Emmanuel, BLAHI Adelaïde Nadia, Coulibaly Founzégué Amadou and Kouakou Koffi

Abstract:
Objective: to study the acute and subacute toxicity of the methanolic extract of Amaranthus viridis leaves on an animal model (Rat). Methods: Acute toxicity was performed according to OECD Guideline 423, by a dose limit of 2000 mg / kg body weight. The observations (during 14 days) concerned the number of deaths, convulsions, sleep and coma. Subacute toxicity was achieved according to the OECD guideline 407. Four groups of 10 rats (5 males and 5 females) received either the dose differences of the methanolic extract (200, 400 and 600 mg/kg) or the vehicle (olive oil). At the end of the treatment, the biochemical, hematological and histological parameters were studied in order to evaluate the toxicity over a long duration of treatment. Conclusion: The results showed that the methanolic extract of Amaranthus viridis administered at the dose 200 and 400 mg/kg is safe at this treatment time on the parameters studied. In contrast, administered at a dose of 600 mg/kg, the methanolic extract revealed signs of toxicity on organ weights, liver and histological markers. Administration at dose of 600 mg/kg is not safe.