Vol. 12, Issue 10 (2023)

Author(s):
Kulumanali Gogoi, Munmi Borah, Palash Deb Nath and Ranima Mishra

Abstract:
Incidence of pumpkin mosaic disease was determined through symptomatology and leaf samples were collected following a roving survey in different pumpkin growing areas of Assam for serological assay; viz., double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and molecular characterization of the virus associated with the disease. Symptomatology revealed various leaf mosaic, mottling, distortion, vein banding, vein clearing and fruit mosaic. DAS-ELISA showed 37 positive reactions out of 84 leaf samples for cucumber mosaic virus (CMV). The total genomic RNA extracted from the infected leaf samples from Jorhat district were subjected to reverse transcription-polymerase chain reaction (RT-PCR) with a pair of primers corresponding to the flanking regions of the cucumber mosaic virus coat protein (CP) gene and yielded the desired amplicon size of 593 bp. Partial sequencing and phylogenetic analysis of the PCR amplicon revealed 93.11 to 99.16 percent sequence homology with other CMV isolates worldwide.