Vol. 12, Issue 10 (2023)

Author(s):
Shanta Sahu, Ashulata Kaushal, Sushma Verma, Ruparani Diwakar, Bhavesh Sahu and Asha

Abstract:
Pathogenicity test were done by mycelial plug and spore suspension method. In mycelial plug method the disease symptoms appeared after three days of inoculation and in spore suspension method the disease symptoms appeared after seven days of inoculation. Based on morphological characters and pathogenicity test, the pathogen was identified as Alternaria sp. The vegetative mycelium in the tissues were hyaline at first, later brown or olivaceous and intercellular. Conidiophore arising singly through the stomata, 0-5(4) septate, with the basal cell sometimes slightly swollen, Olivaceous, unbranched, straight and upright often curled when fasciculate, measured 30-110 (79.50) µm in length and 6-10(8) µm in breadth. Conidia borne in chains up to 6 or more, light to dark olivaceous, solitary straight and slightly flexuous, muriform tapering to beak, with the basal cell rounded, the beak usually flexuous, pale or olivaceous brown, length 517.5 (11.32) µm and 2.5-5(2.8) µm breadth, smooth, 25-80 (49.98) µm, with the beak about onesixth the length of the spore and 5-7.5 (6.5) µm breadth, with 2-5 (3) horizonatal septa and 0-2 (1) vertical septa.