Vol. 12, Issue 11 (2023)

Author(s):
Srijana Pradhan, Kanwar Pal Singh, Reeta Bhatia Dey and Sapna Panwar

Abstract:
In the present investigation, an effort was directed to maintain haploid/di-haploid lines derived through androgenesis in African marigold genotype “Af/w-4” and French marigold genotype “Fr/R-5-2” under in-vitro condition. For successful maintenance it is essential for the shoots and roots to be of optimum quality. Thus, the effect of GA₃ on shoot elongation was studied and recorded to have positive influence on shoot growth. In “Af/w-4”, maximum shoot length increase occurred in MS media+ GA₃ @ 1.00 mg/L both after 15 days (190.62%) and 30 days (312.00%) of shoot inoculation. In “Fr/R-5-2”, GA3 @ 0.50 mg/L recorded maximum shoot length increase (72.88%) after 15 days. For various rooting parameters, IBA was a better as compared to NAA. Half MS media+ IBA @ 1.50 mg/L proved to better for all the rooting attributes in both “Af/w-4” and “Fr/R-5-2” genotypes viz., rooting percentage number of roots per shoot, and root length compared with all the treatment combinations. Marigold micro-shoots did not have difficulty in rooting even in control (media devoid of plant growth regulators) however it was of inferior quality.