Vol. 7, Issue 7 (2018)

Author(s):
Kishore VP and Puttaraju HP

Abstract:
The aim of this study was to evaluate the action of Wolbachia Surface Protein (WSP) on DNA fragmentation in HepG2 cellsexposed to Ethanol. DNA fragmentation in HepG2 cells was caused by exposure of HepG2 cells to Ethanol. Action of Wolbachia Surface Protein (WSP) on DNA fragmentation was evaluated by flow cytometric study of TUNEL assay using FITC-d UTP stain. Flow cytometric study of TUNEL assay analysis of DNA fragmentation using FITC-d UTP stain showed that Ethanol exposure caused DNA fragmentation in HepG2 cells whereas, WSP treatment to Ethanol exposed cells decreased the DNA fragmentation. Thus these observations showed that the treatment with Wolbachia Surface Protein (WSP) suppressed DNA fragmentation in Ethanol exposed HepG2 cells. Finally these findings indicate that Wolbachia Surface Protein (WSP) exhibits suppression of DNA fragmentation. We can conclude that Wolbachia Surface Protein (WSP) exhibits cytoprotective actionagainst DNA fragmentation caused by Ethanol in HepG2 cells by decreasing the DNA fragmentation. This will be of importance for the treatment of Ethanol-associated liver diseases. Thus Wolbachia Surface Protein (WSP) with potential protective action, might be used as an effective therapeutic drug to treat Ethanol-associated liver diseases.