Vol. 7, Issue 7 (2018)

Author(s):
Arundhathi S, Anand Kumar A, Gopala Reddy A, Ravi Kumar Y, Anil Kumar B and Chandravathi T

Abstract:
This study was conducted to know Combined Isoniazid (ISN) and Rifampicin (RIF) induced Hepatotoxicity and emeliorative effects of Withania somnifera and Vitamin E against the toxixcity. Healthy male Wistar rats were divided into four groups consisting of 6 in each.. The experimental study was designed as follows: Group 1-Control, Group II-ISN@50mg/kg body wt + RIF@100mg/kg body wt once daily orally for 21 days, Group IIIISN@50mg/kg body wt + RIF@100mg/kg body wt once daily orally for 21 days + Withania somnifera@1000mg/kg feed daily for 21 days, Group IV-ISN@50mg/kg body wt + RIF@100mg/kg body wt once daily orally for 21 days + vitamin E@300mg/kg feed daily for 21 days. The blood was collected for estimation of serological parameters and tissues were collected for histopathological studies. The serum AST, ALT and GGT activities were increased significantly in group II. Enhanced lipid peroxidation (TBARS and GSH) in tissues was observed indicating oxidative damage. Histopathological changes were noticed significantly in liver of group II rats. The groups III and IV showed significant improvement in all parameters and tissue changes in comparison to group II by the ameliorative effects of Withania somnifera and vitamin E.