Vol. 7, Issue 7 (2018)

Author(s):
J Ruth Sophila, G Dhinakar Raj, K Kumanan, G Sarath Chandra and S Vairamuthu

Abstract:
A preliminary study was conducted to detect the presence of antibiotic residue in milk samples collected in and around Chennai using the microbial inhibition assay with freeze dried vegetative form of Geobacillus stearothermophilus. All the samples were found to be negative for antibiotic residues. Before field testing, the LOD of the commonly used antibiotics namely Enrofloxacin, Ciprofloxacin, Neomycin and Oxytetracycline was determined to be 50, 25,100 and 25 ppb respectively This was much lower than the MRLs for each antibiotic indicating that it is a sensitive assay. The assay would be useful for screening bulk samples as a qualitative test before the confirmatory test using HPLC.