Vol. 12, Issue 11 (2023)

Author(s):
Pothuraju Pradeep Kumar, Dr. C Madhumathi, Dr. M Madhavi, Dr. B Tanuja Priya, Dr. M Paratpara Rao, Dr. M Kavitha and Dr. EK Naik

Abstract:
The study aimed to identify the optimal doses of gamma radiation and Ethyl Methane Sulphonate (EMS) for an effective mutation breeding program in the Acid Lime cultivar Petlur Acid Lime. Seeds of the Petlur Acid Lime cultivar were exposed to gamma irradiation using a Cobalt-60 gamma source. Five treatments, ranging from 20 Gy to 100 Gy, were applied for gamma radiation, and different concentrations of EMS (0.2%, 0.4%, 0.6%, and 0.8%) were used for chemical mutagenesis. Germination and mortality percentages were assessed throughout the study. As the dosage of both chemical and physical mutagens increased, the Petlur Acid Lime cultivar exhibited a gradual rise in mortality percentage. Higher doses were inversely related to germination percentage, resulting in a decrease. The study revealed that higher concentrations of Gamma rays and EMS proved lethal, whereas lower concentrations were safe and ideal for inducing desirable changes. The LD₅₀ doses for gamma rays and EMS were observed in this study at 22.90 Gy and 0.28%, and inferred as 20 Gy and 0.2% EMS.