Vol. 12, Issue 11 (2023)

Author(s):
Vijaysri D and B Narayanaswamy

Abstract:
Probiotics are the live microorganisms that when administered in adequate amounts, confers health benefits on the host. The lactic acid bacteria exist in various environments like water, soil, sewage, plants, fruits, vegetables, as well as in humans and animals. An attempt was made to isolate and evaluate the probiotic characteristics of LAB from pineapple fruits. Totally twenty LAB isolates were obtained from twenty pineapple samples. The isolates were rods, cocci, cocco-bacilli and diplococci in shape and the colonies of the isolates were small, round, white-dull white coloured and creamy with smooth edges. The isolates were Gram positive and catalase negative. They were tolerant and viable at different pH and bile salt conditions. They also showed antimicrobial activity against tested pathogens viz., Escherichia coli and Staphylococcus aureus. The isolates were resistant for most of the antibiotics tested. Hence, the isolates PLAB 10 and PLAB 15 showed potential probiotic properties.