Abstract:This experiment was conducted at Seed Science and Technology Section, Department of Genetics of Plant Breeding, CCSHAU, Hisar Haryana to differentiate wheat varieties based on different chemical tests. Three chemical tests (Standard phenol, Modified phenol and NaOH test) used to differentiate and make group of twenty eight wheat varieties/ genotypes. Among chemical test standard phenol tests and modified phenol test were observed as reliable test for varietal characterization in wheat. it is revealed that these test are less time consuming, easy to perform, economical and reproducible. So can be used as routine tests for varietal identification of wheat genotype in laboratories.