Vol. 12, Issue 11 (2023)

Author(s):
Bushra Khader Ustaad, Dayanand Agsar, Mahadevaswamy and Sirasagikar Reshma N

Abstract:
Enrichment of nutrients in soil by nitrogen fixing bacteria particularly in legumes is an ancient fact know to everyone. Rhizobium sp. plays a key role in agriculture by fixing atmospheric nitrogen to the plants. The present study describes isolation; characterization and screening of Rhizobium sp. 83 Rhizobium sp. were isolated from root nodules of Pigeon pea plant supplemented with flyash. The isolates were characterized morphologically, biochemically, physiologically and further screened for PGPR activity by performing phosphate solubilization test, IAA test, ammonia production, HCN production, siderophore production and nitrate reduction test. Rhizobium isolated BUDR13 and BUDR27 were found to be the highly efficient strains.