Vol. 12, Issue 11 (2023)

Author(s):
Sagar K Jadav, Ajay V Narwade, Lalit Mahatma, Harshal Patil, Gopal Vadodariya and PB Patel

Abstract:
Thirty RAPD primers were utilized to identify the genetic variability of nineteen-finger millet genotypes using Random Amplified Polymorphic DNA (RAPD) molecular markers. A set of 15 RAPD markers that were among the primers used demonstrated polymorphism, and banding patterns were rated as 1 (present) or 0 (missing) in the datasheet. This data was then examined using SAHN clustering and Jaccard's similarity coefficient. The degree of polymorphism among the genotypes of finger millet was evaluated using the number of alleles, PIC value, and heterozygosity for each of the 15 RAPD markers. Fifteen polymorphic RAPD markers had 107 polymorphic loci in total. In 15 RAPD, the number of alleles generated per locus varied from 3 (OPD-13) to 13 (OPA-12). The average PIC value, according to RAPD analysis, was 0.33. In the marker analysis, the 19-finger millet genotypes were divided into two major clusters based on the data produced. Ten genotypes were displayed in RAPD cluster I, and nine genotypes in cluster II.