Vol. 12, Issue 11 (2023)

Author(s):
Manmeet Malpotra, Simrat Pal Singh Saini and Saloni Singla

Abstract:
The present study included the process optimization of different method for preparation of Thiotepa loaded nanoparticles. Ionic gelation method of chitosan nanoparticle preparation was standardized and the physico-chemical characterization was done. Effects of parameters such as chitosan concentration, Chitosan to sodium tripolyphosphate ration and pH of the chitosan solution on the particle size distribution were investigated. The characterization parameter involved estimation of particle size which was 200.37±5.40 nm and PDI o 0.33±0.01 for the drug loaded chitosan nanoparticles. TEM images depicts smooth surface and spherical shape of the nanoparticles. The optimum condition to obtain the smallest nanoparticle were found to be 0.1% chitosan concentration, 4.6 pH of the chitosan solution and 3:1 ratio of chitosan to sodium tripolyphosphate.