Vol. 12, Issue 11 (2023)

Author(s):
Bhyravi BM, Dr. Athani SI, Dr. DP Prakasha, Dr. Anil I Sabarad, Dr. MS Kulkarni, Dr. Nagesh Naik, Dr. Ashok, Dr. Puspha TN and Dr. Yallesh Kumar HS

Abstract:
Bacterial and fungal contaminations are major problems during in vitro culture stages. Therefore, surface sterilization is an obligatory step prior to in vitro culture of any plant tissue and can become a critical point in establishment of certain species, especially when the explant is derived from field grown woody perennial plants. An experiment on “Effect of sterilizing agents on in vitro culture establishment of guava cv. Arka Kiran” was carried out at College of Horticulture, Sirsi, Uttar Kannada, Karnataka during 2021-2023. The study revealed significant influence of different sterilizing agents on initiation response, survival, contamination and health parameters. The nodal explants sterilized with HgCl₂ (0.1%) for five minutes showed maximum initiation of response (100%) and survival (100%), minimum days taken for regeneration (8.35) and contamination (0%) with better health.