Vol. 12, Issue 12 (2023)

Author(s):
Alamgir, Pushpendra Kumar, Mukesh Kumar, Vaishali, MK Yadav and Mukesh Kumar

Abstract:
The Petunia hybrida, an ornamental plant is a member of the Solanaceae family. MADS box transcription factors (TFs) are DNA binding proteins, essential for several plant developmental processes, including the determination of floral organs. In this study, the open reading frame of PhFBP20 gene consists of 651bp nucleotides encoded 216 amino acids which is isolated, cloned and characterized. Phylogenetic analysis showed that PhFBP20 is similar to AGL20 of A. thaliana and SOC1 of S. lycopersicum. The expression analysis revealed that PhFBP20 preferentially expressed in initial bud and leaf at higher rate in comparison to mature bud and open flower which suggest its involvement during floral transition pathways. Further, sequence analysis, identifies variations in the nucleotide (7) and amino acid (4) sequences within the MADS Box domain of the isolated PhFBP20 gene from Petunia hybrida and the FBP20 gene of Petunia axillaris obtained from the NCBI database. No change in nucleotide and amino acid sequence of isolated PhFBP20 from Petunia hybrida and FBP20 of Petunia hybrida gene obtained from NCBI database. These results expand our knowledge of flower formation and aid in the continued research of the MADS-Box gene family in many plant species.