Vol. 12, Issue 12 (2023)

Author(s):
Gajendra GK, Ravi Hunje, SS Chandrashekhar and Ravikumar Hosamani

Abstract:
The green synthesis of nanoparticles has received a lot of interest in recent years. In this context, researchers have explored the use of plant extracts, microorganisms and enzymes from natural resources as an excellent reagents for synthesizing nanoparticles. In this study, synthesis of silver nanoparticles was done through cumin (Cuminum cyminum) seed extract and through reduction method. The bio reduction of silver nitrate (1 mM) using the seed extract of cumin lead to change in colour from light brown to dark brown which confirmed the formation of silver nanoparticles. The synthesized nanoparticles were characterized through UV-Vis, PSA and SEM. Silver nanoparticles showed absorption peak at 420 nm in UV-Vis and average particles size of 64 nm in PSA, respectively. SEM images revealed silver nanoparticles were of spherical shape and smooth surface. Synthesized AgNPs standardized for priming duration in onion seeds. The experiment was conducted on 18 months aged onion seeds cv. Arka Kalyan with twenty nine treatments and three replications. Treatments involved priming seeds at different concentrations of AgNPs solution viz., 25 ppm, 50 ppm, 75 ppm, 100 ppm, 125 ppm, 150 ppm, 200 ppm, 1mM AgNO₃ and control for 1 h, 3 h, 6 h and 12 h respectively. Based on higher germination percentage, root length, shoot length and seedling vigour index- I, the seed priming duration was evaluated. Priming with synthesized AgNPs showed significant variation on seed quality parameters of onion seeds. AgNPs seed priming with 3 hour recorded higher performances for seed quality attributes and considered as best soaking duration for AgNPs priming.