Vol. 12, Issue 12 (2023)

Author(s):
Rajib Das, Jayalekshmy VG, Senpon Ngomle and Prankanu Debnath

Abstract:
The experiment was conducted to study the co-segregation pattern of markers linked to restoring fertility (Rf) gene in HYV of Kerala. Bulk segregation analysis (BSA) was carried out in segregating population of cross between male sterile line UPRI95-17A and Remya, Jayathi, Neeraja and Pavizham to study co-segregation of SSR marker RM 1 and RM 171 associated with Rf3 and Rf4 genes and the trait of restoration of fertility. It was observed that the amplicon size of RM 1 and RM 171 markers in fertile and sterile bulk is similar to their respective fertile and sterile parents. The result indicates that the marker loci and fertility restoration genes Rf3 and Rf4 are tightly linked. Closely linked DNA markers RM1 and RM171 will facilitate effective selection in plant breeding and proper management of genetic purity of hybrid seed.