Abstract:Purpose: The aim of study was to optimize the production of ectoine by the halophilic bacterium strain DSH-3 and characterize the ectoine produced.
Methods: The methods included investigating the effects of various carbon, nitrogen, and amino acid sources on bacterial growth, utilizing Response Surface Methodology (RSM) to optimize ectoine production, and characterizing ectoine through Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) analysis.
Result: The results revealed the optimal conditions for ectoine production and structural similarities between the produced ectoine and the standard compound.
Conclusion: This study contributes to our understanding of ectoine production and its potential applications in biotechnology and biomedicine.