Vol. 7, Issue 8 (2018)

Author(s):
Tanvi, Suman Chaudhary, Rinku Dhanker and Sneh Goyal

Abstract:
An experiment was conducted to evaluate the Lignocellulolytic potential of four fungi isolated from soil samples collected from different ecological niches on malt extract agar medium. Out of four fungi isolated, two isolates showed significant cellulolytic activity with I_CMC varying from 0.745 to 0.805 and all the four isolates showed lignolytic activity with a zone of clearance varying from 0.99 to 1.20. Fungal isolates produced a maximum amount of Lignocellulolytic enzymes at 30 ℃ with HST16 showing maximum CM Case, laccase and lignin peroxidase activity, HST14 showing maximum F Pase activity and HST11 showing maximum manganese peroxidase activity. Lignocellulolytic enzyme production was found more when fungal isolates were grown in medium broth with pH 6 and at stationary condition. Maximum enzyme production occurred on 7^th day of incubation. These results suggested that the environmental conditions could be optimized for growth of Lignocellulolytic fungi and these fungal isolates could be exploited for compost preparation, in paper industry, textile industry and for improvement of soil texture and fertility.