Vol. 12, Issue 12 (2023)

Author(s):
Pooja PS, Shadab M Khatib, Kopparthi Amrutha Valli Sindhura, Bharath M, Kavya SS, Ningaraju TM, KS Shankarappa and CN Lakshminarayana Reddy

Abstract:
CMV (cucumber mosaic virus) is the most important and devastating virus infecting chilli (Capsicum annuum. L). Leaf sample from chilli plants expressing systemic mosaic and mottling symptoms, typical to CMV infection was collected and designated as CMV PS1 isolate. The CMV isolate was mechanically inoculated to chilli and Nicotiana glutinosa plants. For molecular characterization, total RNA was isolated from infected plants and used for carrying out RT-PCR with CMV coat protein (CP) specific primers. PCR amplification generated an expected CP amplicon of 1.1 kb, which was further cloned and sequenced. The CMV CP nucleotide sequence showed 93.91 to 99.19 per cent identity with other CMV isolates infecting different crops worldwide. The sequence demarcation graph and phylogenetic analysis showed close association of CMV PS1 isolate with CMV isolate Ho (MN340998.1) belonging to subgroup IB prevailing in Indian conditions.