Vol. 12, Issue 12 (2023)

Author(s):
PS Shinde, RS Deshpande, AV Mane, SV Sawardekar and AD Rane

Abstract:
The present investigation on “In vitro shoot development from callus and other explants in Teak (Tectona grandis Linn. F.)” was carried out at the Plant Biotechnology Centre, College of Agriculture, Dapoli, Dist. Ratnagiri 415 712 (Maharashtra). Among the matured and juvenile explants used, apical buds and nodes from juvenile plants were found best for shoot induction. T₁₁ (Ethyl Alcohol 70% for 10 sec. + HgCl₂ 0.05% for 5 min.) achieved the highest aseptic culture establishment rate (70.60%) and the highest survival rate (72.00%) for surface sterilization of these explants. Browning of the media was prevented by using PVP (100 mg l^-1) as an antioxidant and dark incubation for three days, resulted in aseptic cultures. Among the various treatments, the MS medium with 2.0 mgl^-1 BAP shown the best for teak explant establishment and shoot induction. In MS media supplemented with 1.0 mg l^-1 BAP and 2.0 mg l^-1 NAA, callus induction was better in leaf explants of young teak plants while shoot induction was better in apical buds and nodes.